Gluconic acid is the carboxylic acid formed by the oxidation of the first carbon of glucose with antiseptic and chelating properties. Gluconic acid, found abundantly in plant, honey and wine, can be prepared by fungal fermentation process commercially. Gluconic acid and its salts have been used for many applications such as house hold cleaner in industrial cleaner, paints dyes for metal finishing , as paper textile auxiliaries and in water treatment. Due to low toxicity low corrosivity, and capability of forming water soluble complexes with divalent and trivalent metal ions, gluconic acid is widely used in many industries. The formation of gluconic acid is different from most other organic acids, since it is formed outside the cytoplasmic membrane by the enzyme glucose oxidase.