Nitrilase are involved in natural product in biosynthesis and post translational modifications in plants, animals, fungi and certain prokaryotes. Nitrilases can also be used as catalysts in preparative organic chemistry. Nitrilases have been used for the resolution of racemic mixtures. Nitrilases are derived from the bacteria, filamentous fungi, yeast and plants. Nitrilases are enzyme that catalyse the hydrolysis of nitrile compounds to the corresponding carboxylic acid and ammonia, and have a wide range of industrial and biotechnology application including synthesis of industrially important carboxylic acid and bioremediation of cyanide and toxic.